› 4tpw

X-ray diffraction
1.5Å resolution

The co-complex structure of the translation initiation factor eIF4E with the inhibitor 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G

Released:
DOI: 10.2210/pdb4tpw/pdb
PDB entry 4tpw coloured by chain, front view.
PDB entry 4tpw coloured by chain, side view.
PDB entry 4tpw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the eukaryotic translation initiation factor eIF4E in complex with 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G.
Papadopoulos E, Jenni S, Kabha E, Takrouri KJ, Yi T, Salvi N, Luna RE, Gavathiotis E, Mahalingam P, Arthanari H, Rodriguez-Mias R, Yefidoff-Freedman R, Aktas BH, Chorev M, Halperin JA, Wagner G
Proc Natl Acad Sci U S A 111 E3187-95 (2014)
PMID: 25049413

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 22.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06730 (Residues: 28-217; Coverage: 88%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

3 bound ligands:
Ligand MGP 2 x MGP
Ligand 33R 1 x 33R
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 39.34Å b: 73.2Å c: 65.72Å
α: 90° β: 106.25° γ: 90°
R-values:
R R work R free
0.172 0.172 0.193
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Targeting the translation machinery in cancer.
Bhat et al. (2015)
16 more

10 mentions without citation

Molecular mechanism of the dual activity of 4EGI-1: Dissociating eIF4G from eIF4E but stabilizing the binding of unphosphorylated 4E-BP1.
Sekiyama et al. (2015)
9 more