› 4tl6

X-ray diffraction
1.76Å resolution

Crystal structure of N-terminal domain of KaiC

Released:
DOI: 10.2210/pdb4tl6/pdb
PDB entry 4tl6 coloured by chain, front view.
PDB entry 4tl6 coloured by chain, side view.
PDB entry 4tl6 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Circadian rhythms. Atomic-scale origins of slowness in the cyanobacterial circadian clock.
Abe J, Hiyama TB, Mukaiyama A, Son S, Mori T, Saito S, Osako M, Wolanin J, Yamashita E, Kondo T, Akiyama S
Science 349 312-6 (2015)
PMID: 26113637

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 253 amino acids
Theoretical weight: 28.38 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF4 (Residues: 1-253; Coverage: 49%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ANP 3 x ANP
Ligand MG 3 x MG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 134.823Å b: 81.057Å c: 95.64Å
α: 90° β: 132.87° γ: 90°
R-values:
R R work R free
0.17 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Circadian Oscillators: Around the Transcription-Translation Feedback Loop and on to Output.
Hurley et al. (2016)
15 more