› 4rv9

X-ray diffraction
2.2Å resolution

Crystal structure of MtmC in complex with SAH

Released:
DOI: 10.2210/pdb4rv9/pdb
PDB entry 4rv9 coloured by chain, front view.
PDB entry 4rv9 coloured by chain, side view.
PDB entry 4rv9 coloured by chain, top view.
Source organism: Streptomyces argillaceus
Primary publication:
Structural insight into MtmC, a bifunctional ketoreductase-methyltransferase involved in the assembly of the mithramycin trisaccharide chain.
Chen JM, Hou C, Wang G, Tsodikov OV, Rohr J
Biochemistry 54 2481-9 (2015)
PMID: 25587924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-mycarose 3-C-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 46.94 KDa
Source organism: Streptomyces argillaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q194Q4 (Residues: 1-420; Coverage: 100%)
Gene name: mtmC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand SAH 1 x SAH
Ligand ACT 1 x ACT
Ligand CL 1 x CL
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4122
Unit cell:
a: 134.245Å b: 134.245Å c: 130.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.241
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Selvamicin, an atypical antifungal polyene from two alternative genomic contexts.
Van Arnam et al. (2016)
2 more