› 4r52

X-ray diffraction
1.53Å resolution

1.5 angstrom crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from Cupriavidus metallidurans

Released:
DOI: 10.2210/pdb4r52/pdb
PDB entry 4r52 coloured by chain, front view.
PDB entry 4r52 coloured by chain, side view.
PDB entry 4r52 coloured by chain, top view.
Source organism: Cupriavidus metallidurans CH34
Entry authors: Geng J, Gumpper RH, Huo L, Liu A

Function and Biology Details

Reaction catalysed: 
3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-172947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyanthranilate 3,4-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.06 KDa
Source organism: Cupriavidus metallidurans CH34
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1LCS4 (Residues: 1-174; Coverage: 100%)
Gene names: Rmet_5193, nbaC
Sequence domains: 3-hydroxyanthranilic acid dioxygenase
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6522
Unit cell:
a: 58.32Å b: 58.32Å c: 231.849Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.197 0.214
Expression system: Escherichia coli BL21(DE3)

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