› 4r4f

X-ray diffraction
3.51Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211125 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Surface protein gp120 Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 41.7 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P35961 (Residues: 21-479; Coverage: 45%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
Antibody 2.2c LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 209 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody 2.2c heavy CHAIN Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
M48U1 peptide Chain: R
Molecule details ›
Chain: R
Length: 28 amino acids
Theoretical weight: 3.06 KDa

Ligands and Environments

3 bound ligands:
Ligand NAG 8 x NAG
Ligand BU3 3 x BU3
Ligand SO4 2 x SO4
3 modified residues:
Ligand OAS 1 x OAS
Ligand DPR 1 x DPR
Ligand U2X 1 x U2X

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: F222
Unit cell:
a: 158.321Å b: 171.23Å c: 227.974Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.25 0.295
Expression system: Escherichia coli

Quick links

Citations

13 review citations

HIV antibodies for treatment of HIV infection.
Margolis et al. (2017)
12 more

4 mentions without citation

Conformational Masking and Receptor-Dependent Unmasking of Highly Conserved Env Epitopes Recognized by Non-Neutralizing Antibodies That Mediate Potent ADCC against HIV-1.
Lewis et al. (2015)
3 more