› 4qry

X-ray diffraction
2.2Å resolution

the ground state and the N intermediate of pharaonis halorhodopsin in complex with bromide ion

Released:
DOI: 10.2210/pdb4qry/pdb
PDB entry 4qry coloured by chain, front view.
PDB entry 4qry coloured by chain, side view.
PDB entry 4qry coloured by chain, top view.
Source organism: Natronomonas pharaonis
Primary publication:
Crystal structures of the L1, L2, N, and O states of pharaonis halorhodopsin.
Kouyama T, Kawaguchi H, Nakanishi T, Kubo H, Murakami M
Biophys J 108 2680-90 (2015)
PMID: 26039169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chains: A, B, C, E, F, G
Molecule details ›
Chains: A, B, C, E, F, G
Length: 291 amino acids
Theoretical weight: 30.98 KDa
Source organism: Natronomonas pharaonis
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

4 bound ligands:
Ligand RET 6 x RET
Ligand BR 12 x BR
Ligand BNG 5 x BNG
Ligand L2P 6 x L2P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 153.36Å b: 98.3Å c: 100.53Å
α: 90° β: 128.13° γ: 90°
R-values:
R R work R free
0.214 0.214 0.243

Quick links

Citations

3 review citations

Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.
Inoue K. (2021)
2 more