› 4qoz

X-ray diffraction
2.3Å resolution

Crystal structure of the histone mRNA stem-loop, stem-loop binding protein (phosphorylated), and 3'hExo ternary complex

Released:
DOI: 10.2210/pdb4qoz/pdb
PDB entry 4qoz coloured by chain, front view.
PDB entry 4qoz coloured by chain, side view.
PDB entry 4qoz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanisms for the regulation of histone mRNA stem-loop-binding protein by phosphorylation.
Zhang J, Tan D, DeRose EF, Perera L, Dominski Z, Marzluff WF, Tong L, Hall TM
Proc Natl Acad Sci U S A 111 E2937-46 (2014)
PMID: 25002523

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-172013 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
3'-5' exoribonuclease 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 303 amino acids
Theoretical weight: 35.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IV48 (Residues: 55-349; Coverage: 85%)
Gene names: 3'EXO, ERI1, THEX1
Sequence domains:
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
Histone RNA hairpin-binding protein Chain: C
Molecule details ›
Chain: C
Length: 120 amino acids
Theoretical weight: 14.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q14493 (Residues: 125-223; Coverage: 37%)
Gene names: HBP, SLBP
Sequence domains: Histone RNA hairpin-binding protein RNA-binding domain
Structure domains: Histone RNA hairpin-binding protein RNA-binding domain
histone mRNA stem-loop Chains: A, D
Molecule details ›
Chains: A, D
Length: 26 nucleotides
Theoretical weight: 8.22 KDa
Sequence domains: Histone 3' UTR stem-loop

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 82.107Å b: 91.505Å c: 128.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda

Quick links

Citations

6 review citations

RNA Structures as Mediators of Neurological Diseases and as Drug Targets.
Bernat et al. (2015)
5 more

10 mentions without citation

Structural basis for regulation of RNA-binding proteins by phosphorylation.
Thapar R. (2015)
9 more