› 4qnc

X-ray diffraction
2.39Å resolution

Crystal structure of a SemiSWEET in an occluded state

Released:
DOI: 10.2210/pdb4qnc/pdb
PDB entry 4qnc coloured by chain, front view.
PDB entry 4qnc coloured by chain, side view.
PDB entry 4qnc coloured by chain, top view.
Source organism: Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
Primary publication:
Structures of bacterial homologues of SWEET transporters in two distinct conformations.
Xu Y, Tao Y, Cheung LS, Fan C, Chen LQ, Xu S, Perry K, Frommer WB, Feng L
Nature 515 448-452 (2014)
PMID: 25186729

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar transporter SemiSWEET Chains: A, B
Molecule details ›
Chains: A, B
Length: 93 amino acids
Theoretical weight: 10.72 KDa
Source organism: Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0SR19 (Residues: 1-85; Coverage: 100%)
Gene name: LEPBI_I1613
Sequence domains: PQ loop repeat
Structure domains: Monooxygenase

Ligands and Environments

2 bound ligands:
Ligand OLC 4 x OLC
Ligand MYS 4 x MYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D, APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 36.907Å b: 51.833Å c: 46.963Å
α: 90° β: 94.87° γ: 90°
R-values:
R R work R free
0.267 0.266 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
27 more

11 mentions without citation

GLUT, SGLT, and SWEET: Structural and mechanistic investigations of the glucose transporters.
Deng et al. (2016)
10 more