› 4qjd

X-ray diffraction
3.1Å resolution

Crystal Structure of Twister with the Nucleotide 5'- to the Cleavage Site Disordered at 3.1 A Resolution

Released:
DOI: 10.2210/pdb4qjd/pdb
PDB entry 4qjd coloured by chain, front view.
PDB entry 4qjd coloured by chain, side view.
PDB entry 4qjd coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme.
Eiler D, Wang J, Steitz TA
Proc Natl Acad Sci U S A 111 13028-33 (2014)
PMID: 25157168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196861 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
Twister RNA sequence Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 26 nucleotides
Theoretical weight: 8.16 KDa
Source organism: Synthetic construct
Expression system: Not provided
Twister RNA sequence Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 47 nucleotides
Theoretical weight: 15.47 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
1 modified residue:
Ligand GTP 4 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 70.91Å b: 53.07Å c: 106.75Å
α: 90° β: 106.86° γ: 90°
R-values:
R R work R free
0.195 0.19 0.287
Expression system: Not provided

Quick links

Citations

11 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
10 more

5 mentions without citation

In-line alignment and Mg²⁺ coordination at the cleavage site of the env22 twister ribozyme.
Ren et al. (2014)
4 more