› 4q3h

X-ray diffraction
1.44Å resolution

The crystal structure of NHERF1 PDZ2 CXCR2 complex revealed by the NHERF1 CXCR2 chimeric protein

Released:
DOI: 10.2210/pdb4q3h/pdb
PDB entry 4q3h coloured by chain, front view.
PDB entry 4q3h coloured by chain, side view.
PDB entry 4q3h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the NHERF1 PDZ2 domain in complex with the chemokine receptor CXCR2 reveals probable modes of PDZ2 dimerization.
Holcomb J, Jiang Y, Guan X, Trescott L, Lu G, Hou Y, Wang S, Brunzelle J, Sirinupong N, Li C, Yang Z
Biochem Biophys Res Commun 448 169-74 (2014)
PMID: 24768637

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 9.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14745 (Residues: 150-235; Coverage: 24%)
Gene names: NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 32.751Å b: 55.349Å c: 45.226Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.167 0.165 0.195
Expression system: Escherichia coli

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Citations

2 review citations

CXCR2 Receptor: Regulation of Expression, Signal Transduction, and Involvement in Cancer.
Korbecki et al. (2022)
1 more

2 mentions without citation

Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport.
Zhang et al. (2019)
1 more