› 4q0i

X-ray diffraction
1.74Å resolution

Deinococcus radiodurans BphP PAS-GAF D207A mutant

Released:
DOI: 10.2210/pdb4q0i/pdb
PDB entry 4q0i coloured by chain, front view.
PDB entry 4q0i coloured by chain, side view.
PDB entry 4q0i coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Crystallographic and electron microscopic analyses of a bacterial phytochrome reveal local and global rearrangements during photoconversion.
Burgie ES, Wang T, Bussell AN, Walker JM, Li H, Vierstra RD
J Biol Chem 289 24573-87 (2014)
PMID: 25006244

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 36.98 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IPA 2 x IPA
Ligand LBV 1 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 87.497Å b: 51.327Å c: 80.368Å
α: 90° β: 115.75° γ: 90°
R-values:
R R work R free
0.163 0.161 0.195
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Phytochromes: an atomic perspective on photoactivation and signaling.
Burgie et al. (2014)
6 more

1 mention without citation

Crystallographic and electron microscopic analyses of a bacterial phytochrome reveal local and global rearrangements during photoconversion.
Burgie et al. (2014)