› 4pxl

X-ray diffraction
2.25Å resolution

Structure of Zm ALDH2-3 (RF2C) in complex with NAD

Released:
DOI: 10.2210/pdb4pxl/pdb
PDB entry 4pxl coloured by chain, front view.
PDB entry 4pxl coloured by chain, side view.
PDB entry 4pxl coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Role and structural characterization of plant aldehyde dehydrogenases from family 2 and family 7.
Končitíková R, Vigouroux A, Kopečná M, Andree T, Bartoš J, Šebela M, Moréra S, Kopečný D
Biochem J 468 109-23 (2015)
PMID: 25734422

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186064 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aldehyde dehydrogenase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 517 amino acids
Theoretical weight: 55.85 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8S532 (Residues: 2-502; Coverage: 100%)
Gene names: 541913, ZEAMMB73_Zm00001d044339, rf2c
Sequence domains: Aldehyde dehydrogenase family
Structure domains:
Aldehyde dehydrogenase domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 517 amino acids
Theoretical weight: 55.83 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8S532 (Residues: 2-502; Coverage: 100%)
Gene names: 541913, ZEAMMB73_Zm00001d044339, rf2c
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NAD 2 x NAD
Ligand EDO 9 x EDO
Ligand PEG 4 x PEG
Ligand NA 2 x NA
Ligand CA 1 x CA
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 109.86Å b: 126.08Å c: 78.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Branched-Chain Volatiles in Fruit: A Molecular Perspective.
Bizzio et al. (2021)
1 more

4 mentions without citation

Active Sites of Reduced Epidermal Fluorescence1 (REF1) Isoforms Contain Amino Acid Substitutions That Are Different between Monocots and Dicots.
Missihoun et al. (2016)
3 more