› 4prq

X-ray diffraction
1.72Å resolution

CRYSTAL STRUCTURE OF HEN EGG-WHITE LYSOZYME IN COMPLEX WITH SCLX4 AT 1.72 A RESOLUTION

Released:
DOI: 10.2210/pdb4prq/pdb
PDB entry 4prq coloured by chain, front view.
PDB entry 4prq coloured by chain, side view.
PDB entry 4prq coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Protein assembly mediated by sulfonatocalix[4]arene.
McGovern RE, McCarthy AA, Crowley PB
Chem Commun (Camb) 50 10412-5 (2014)
PMID: 25068633

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

5 bound ligands:
Ligand T3Y 5 x T3Y
Ligand PG4 3 x PG4
Ligand P6G 1 x P6G
Ligand PG6 1 x PG6
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 44.01Å b: 81.749Å c: 72.1Å
α: 90° β: 105.37° γ: 90°
R-values:
R R work R free
0.188 0.186 0.222
Expression system: Escherichia coli

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Citations

6 review citations

Moulding calixarenes for biomacromolecule targeting.
Giuliani et al. (2015)
5 more

5 mentions without citation

Atomic Details of Carbon-Based Nanomolecules Interacting with Proteins.
Di Costanzo et al. (2020)
4 more