› 4pll

X-ray diffraction
2.6Å resolution

Structure of the chromodaomain of MRG2 in complex with H3K36me3

Released:
DOI: 10.2210/pdb4pll/pdb
PDB entry 4pll coloured by chain, front view.
PDB entry 4pll coloured by chain, side view.
PDB entry 4pll coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Bu Z, Yu Y, Li Z, Liu Y, Jiang W, Huang Y, Dong AW
PLoS Genet 10 e1004617 (2014)
PMID: 25211338

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein MRG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 9.12 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4V3E2 (Residues: 51-123; Coverage: 22%)
Gene names: At1g02740, F22D16.25, MRG2
Sequence domains: RNA binding activity-knot of a chromodomain
Structure domains: SH3 type barrels.
Histone H3.3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P59169 (Residues: 32-42; Coverage: 8%)
Gene names: At4g40030, At4g40040, At5g10980, HTR4, HTR5, HTR8, T30N20_250, T5J17.200, T5J17.210, T5K6.6

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P61
Unit cell:
a: 109.157Å b: 109.157Å c: 29.977Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.24 0.279
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Tug of war: adding and removing histone lysine methylation in Arabidopsis.
Xiao et al. (2016)
14 more

2 mentions without citation

Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Bu et al. (2014)
1 more