4p01 Summary


Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with N-[(4-cyanophenyl)methyl]methanethioamide

The structure was published by Pantouris, G. and Lolis, E., in in a paper entitled "to be published" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.07 Å and deposited in 2014.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Macrophage migration inhibitory factor.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%
B Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%
C Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P14174 (2 - 115) Macrophage migration inhibitory factor Homo sapiens

Chain Sequence family (Pfam)
A, B, C (P14174) PF01187: Macrophage migration inhibitory factor (MIF)search

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
A, B, C (P14174) vesiclesearch extracellular vesicular exosomesearch extracellular regionsearch extracellular spacesearch cell surfacesearch nucleoplasmsearch cytoplasmsearch positive regulation of peptidyl-tyrosine phosphorylationsearch negative regulation of DNA damage response, signal transduction by p53 class mediatorsearch positive regulation of peptidyl-serine phosphorylationsearch inflammatory responsesearch positive regulation of arachidonic acid secretionsearch positive regulation of chemokine (C-X-C motif) ligand 2 productionsearch positive regulation of ERK1 and ERK2 cascadesearch positive regulation of fibroblast proliferationsearch regulation of cell proliferationsearch positive regulation of B cell proliferationsearch positive chemotaxissearch negative regulation of cell agingsearch regulation of macrophage activationsearch immune system processsearch negative regulation of cell cycle arrestsearch positive regulation of phosphorylationsearch negative regulation of apoptotic processsearch negative regulation of myeloid cell apoptotic processsearch cell surface receptor signaling pathwaysearch DNA damage response, signal transduction by p53 class mediatorsearch negative regulation of cellular protein metabolic processsearch positive regulation of protein phosphorylationsearch innate immune responsesearch carboxylic acid metabolic processsearch cell proliferationsearch positive regulation of MAP kinase activitysearch negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatorsearch negative regulation of mature B cell apoptotic processsearch positive regulation of prostaglandin secretion involved in immune responsesearch positive regulation of protein kinase A signalingsearch cell agingsearch protein homotrimerizationsearch positive regulation of myeloid leukocyte cytokine production involved in immune responsesearch negative regulation of gene expressionsearch positive regulation of lipopolysaccharide-mediated signaling pathwaysearch prostaglandin biosynthetic processsearch positive regulation of cytokine secretionsearch phenylpyruvate tautomerase activitysearch cytokine activitysearch receptor bindingsearch chemoattractant activitysearch isomerase activitysearch dopachrome isomerase activitysearch cytokine receptor bindingsearch protein bindingsearch

Chain InterPro annotation
A, B, C Macrophage migration inhibitory factorsearch Tautomerase/MIF superfamilysearch Macrophage migration inhibitory factor, conserved sitesearch