4p01 Summary


Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with N-[(4-cyanophenyl)methyl]methanethioamide

The structure was published by Pantouris, G. and Lolis, E., in in a paper entitled "to be published" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.07 Å and deposited in 2014.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Macrophage migration inhibitory factor.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%
B Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%
C Macrophage migration inhibitory factor P14174 (2-115) (MIF_HUMAN)search Homo sapienssearch 98% 114 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P14174 (2 - 115) Macrophage migration inhibitory factor Homo sapiens

Chain Sequence family (Pfam)
A, B, C (P14174) PF01187: Macrophage migration inhibitory factor (MIF)search

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B, C (P14174) positive regulation of arachidonic acid secretionsearch negative regulation of gene expressionsearch positive regulation of fibroblast proliferationsearch negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatorsearch regulation of cell proliferationsearch inflammatory responsesearch positive regulation of ERK1 and ERK2 cascadesearch positive chemotaxissearch negative regulation of DNA damage response, signal transduction by p53 class mediatorsearch positive regulation of peptidyl-tyrosine phosphorylationsearch cell surface receptor signaling pathwaysearch carboxylic acid metabolic processsearch negative regulation of cellular protein metabolic processsearch positive regulation of prostaglandin secretion involved in immune responsesearch positive regulation of peptidyl-serine phosphorylationsearch negative regulation of mature B cell apoptotic processsearch protein homotrimerizationsearch prostaglandin biosynthetic processsearch negative regulation of apoptotic processsearch positive regulation of cytokine secretionsearch positive regulation of protein kinase A signalingsearch negative regulation of cell agingsearch positive regulation of lipopolysaccharide-mediated signaling pathwaysearch positive regulation of myeloid leukocyte cytokine production involved in immune responsesearch positive regulation of chemokine (C-X-C motif) ligand 2 productionsearch negative regulation of cell cycle arrestsearch positive regulation of phosphorylationsearch positive regulation of MAP kinase activitysearch positive regulation of B cell proliferationsearch innate immune responsesearch regulation of macrophage activationsearch cell agingsearch immune system processsearch negative regulation of myeloid cell apoptotic processsearch positive regulation of protein phosphorylationsearch DNA damage response, signal transduction by p53 class mediatorsearch cell proliferationsearch protein bindingsearch dopachrome isomerase activitysearch chemoattractant activitysearch cytokine activitysearch phenylpyruvate tautomerase activitysearch cytokine receptor bindingsearch isomerase activitysearch receptor bindingsearch cytoplasmsearch nucleoplasmsearch extracellular regionsearch extracellular spacesearch extracellular vesicular exosomesearch vesiclesearch cell surfacesearch

Chain InterPro annotation
A, B, C Macrophage migration inhibitory factorsearch Tautomerase/MIF superfamilysearch Macrophage migration inhibitory factor, conserved sitesearch