› 4ozn

X-ray diffraction
2.6Å resolution

GlnK2 from Haloferax mediterranei complexed with ATP

Released:
DOI: 10.2210/pdb4ozn/pdb
PDB entry 4ozn coloured by chain, front view.
PDB entry 4ozn coloured by chain, side view.
PDB entry 4ozn coloured by chain, top view.
Source organism: Haloferax mediterranei ATCC 33500
Primary publication:
The structure of a PII signaling protein from a halophilic archaeon reveals novel traits and high-salt adaptations.
Palanca C, Pedro-Roig L, Llácer JL, Camacho M, Bonete MJ, Rubio V
FEBS J 281 3299-314 (2014)
PMID: 24946894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein GlnK2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 143 amino acids
Theoretical weight: 15.02 KDa
Source organism: Haloferax mediterranei ATCC 33500
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8ZYW1 (Residues: 1-123; Coverage: 100%)
Gene names: BM92_08825, C439_09930, E6P09_03555, HFX_0092, glnK2
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ATP 3 x ATP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3121
Unit cell:
a: 110.717Å b: 110.717Å c: 76.949Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.217 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Sensory properties of the PII signalling protein family.
Forchhammer et al. (2016)
1 more

1 mention without citation

The structure of a PII signaling protein from a halophilic archaeon reveals novel traits and high-salt adaptations.
Palanca et al. (2014)