› 4ozj

X-ray diffraction
1.45Å resolution

GlnK2 from Haloferax mediterranei complexed with ADP

Released:
DOI: 10.2210/pdb4ozj/pdb
PDB entry 4ozj coloured by chain, front view.
PDB entry 4ozj coloured by chain, side view.
PDB entry 4ozj coloured by chain, top view.
Source organism: Haloferax mediterranei ATCC 33500
Primary publication:
The structure of a PII signaling protein from a halophilic archaeon reveals novel traits and high-salt adaptations.
Palanca C, Pedro-Roig L, Llácer JL, Camacho M, Bonete MJ, Rubio V
FEBS J 281 3299-314 (2014)
PMID: 24946894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein GlnK2 Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 15.02 KDa
Source organism: Haloferax mediterranei ATCC 33500
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8ZYW1 (Residues: 1-123; Coverage: 100%)
Gene names: BM92_08825, C439_09930, E6P09_03555, HFX_0092, glnK2
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: R32
Unit cell:
a: 89.185Å b: 89.185Å c: 96.349Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Sensory properties of the PII signalling protein family.
Forchhammer et al. (2016)
1 more

2 mentions without citation

The structure of a PII signaling protein from a halophilic archaeon reveals novel traits and high-salt adaptations.
Palanca et al. (2014)
1 more