› 4oso

X-ray diffraction
2.5Å resolution

The crystal structure of landomycin C-6 ketoreductase LanV with bound NADP and rabelomycin

Released:
DOI: 10.2210/pdb4oso/pdb
PDB entry 4oso coloured by chain, front view.
PDB entry 4oso coloured by chain, side view.
PDB entry 4oso coloured by chain, top view.
Source organism: Streptomyces cyanogenus
Primary publication:
Structure-based engineering of angucyclinone 6-ketoreductases.
Patrikainen P, Niiranen L, Thapa K, Paananen P, Tähtinen P, Mäntsälä P, Niemi J, Metsä-Ketelä M
Chem Biol 21 1381-1391 (2014)
PMID: 25200607

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reductase homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 27.5 KDa
Source organism: Streptomyces cyanogenus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZGC1 (Residues: 2-253; Coverage: 100%)
Gene name: lanV
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAP 2 x NAP
Ligand 2V4 2 x 2V4
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41212
Unit cell:
a: 91.99Å b: 91.99Å c: 106.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.174 0.235
Expression system: Escherichia coli

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Citations

2 review citations

New insights into bacterial type II polyketide biosynthesis.
Zhang et al. (2017)
1 more

7 mentions without citation

Galactosaminoglycans: Medical Applications and Drawbacks.
Pomin et al. (2019)
6 more