› 4ogp

X-ray diffraction
2.15Å resolution

Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator (Space group : P21)

Released:
DOI: 10.2210/pdb4ogp/pdb
PDB entry 4ogp coloured by chain, front view.
PDB entry 4ogp coloured by chain, side view.
PDB entry 4ogp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment.
Fourati Z, Kolesnikova O, Back R, Keller J, Charenton C, Taverniti V, Plesse CG, Lazar N, Durand D, van Tilbeurgh H, Séraphin B, Graille M
PLoS One 9 e96828 (2014)
PMID: 24830408

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deadenylation-dependent mRNA-decapping factor PAT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 38.48 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P25644 (Residues: 473-796; Coverage: 41%)
Gene names: MRT1, PAT1, YCR077C, YCR77C
Sequence domains: Topoisomerase II-associated protein PAT1

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 55.12Å b: 88.46Å c: 67.91Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.207 0.204 0.261
Expression system: Escherichia coli

Quick links

Citations

3 review citations

mRNA decapping: finding the right structures.
Charenton et al. (2018)
2 more

1 mention without citation

The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment.
Fourati et al. (2014)