› 4ofn

X-ray diffraction
2.69Å resolution

Monoclinic NaGST1

Released:
DOI: 10.2210/pdb4ofn/pdb
PDB entry 4ofn coloured by chain, front view.
PDB entry 4ofn coloured by chain, side view.
PDB entry 4ofn coloured by chain, top view.
Source organism: Necator americanus
Primary publication:
Structure of glutathione S-transferase 1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione.
Asojo OA, Ceccarelli C
Acta Crystallogr F Struct Biol Commun 70 1162-6 (2014)
PMID: 25195885

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 23.71 KDa
Source organism: Necator americanus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: D3U1A5 (Residues: 1-206; Coverage: 100%)
Gene name: gst-1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GSH 2 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: C2
Unit cell:
a: 84.554Å b: 45.213Å c: 113.763Å
α: 90° β: 112.11° γ: 90°
R-values:
R R work R free
0.294 0.292 0.33
Expression system: Komagataella pastoris

Quick links

Citations

4 citation in other articles

Serendipitous SAD Solution for DMSO-Soaked SOCS2-ElonginC-ElonginB Crystals Using Covalently Incorporated Dimethylarsenic: Insights into Substrate Receptor Conformational Flexibility in Cullin RING Ligases.
Gadd et al. (2015)
3 more

1 mention without citation

Structure of glutathione S-transferase 1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione.
Asojo et al. (2014)