› 4ocg

X-ray diffraction
2.75Å resolution

Structure of the Shewanella loihica PV-4 NADH-dependent persulfide reductase F161A Mutant

Released:
DOI: 10.2210/pdb4ocg/pdb
PDB entry 4ocg coloured by chain, front view.
PDB entry 4ocg coloured by chain, side view.
PDB entry 4ocg coloured by chain, top view.
Source organism: Shewanella loihica PV-4
Primary publication:
Characterization of the mechanism of the NADH-dependent polysulfide reductase (Npsr) from Shewanella loihica PV-4: formation of a productive NADH-enzyme complex and its role in the general mechanism of NADH and FAD-dependent enzymes.
Lee KH, Humbarger S, Bahnvadia R, Sazinsky MH, Crane EJ
Biochim Biophys Acta 1844 1708-17 (2014)
PMID: 24981797

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-107208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodanese domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 62.35 KDa
Source organism: Shewanella loihica PV-4
Expression system: Escherichia coli
UniProt:
  • Canonical: A3QAV3 (Residues: 1-566; Coverage: 100%)
Gene name: Shew_0729
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand COA 2 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3
Unit cell:
a: 133.71Å b: 133.71Å c: 84.317Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.125 0.122 0.173
Expression system: Escherichia coli

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Citations

1 review citation

H2S and reactive sulfur signaling at the host-bacterial pathogen interface.
Walsh et al. (2020)
2 other articles