› 4o5a

X-ray diffraction
1.78Å resolution

The crystal structure of a LacI family transcriptional regulator from Bifidobacterium animalis subsp. lactis DSM 10140

Released:
DOI: 10.2210/pdb4o5a/pdb
PDB entry 4o5a coloured by chain, front view.
PDB entry 4o5a coloured by chain, side view.
PDB entry 4o5a coloured by chain, top view.
Source organism: Bifidobacterium animalis subsp. lactis DSM 10140
Entry authors: Tan K, Li H, Endres M, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LacI family transcription regulator Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 37.82 KDa
Source organism: Bifidobacterium animalis subsp. lactis DSM 10140
Expression system: Escherichia coli BL21(DE3)
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 2 x SO4
2 modified residues:
Ligand MSE 12 x MSE
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 65.214Å b: 88.913Å c: 60.524Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.167 0.166 0.196
Expression system: Escherichia coli BL21(DE3)

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