› 4nb8

X-ray diffraction
2.01Å resolution

Oxygenase with Ile262 replaced by Leu and ferredoxin complex of carbazole 1,9a-dioxygenase

Released:
DOI: 10.2210/pdb4nb8/pdb
PDB entry 4nb8 coloured by chain, front view.
PDB entry 4nb8 coloured by chain, side view.
PDB entry 4nb8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the divergent oxygenation reactions catalyzed by the rieske nonheme iron oxygenase carbazole 1,9a-dioxygenase.
Inoue K, Usami Y, Ashikawa Y, Noguchi H, Umeda T, Yamagami-Ashikawa A, Horisaki T, Uchimura H, Terada T, Nakamura S, Shimizu K, Habe H, Yamane H, Fujimoto Z, Nojiri H
Appl Environ Microbiol 80 2821-32 (2014)
PMID: 24584240

Function and Biology Details

Reaction catalysed: 
9H-carbazole + NAD(P)H + O(2) = 2'-aminobiphenyl-2,3-diol + NAD(P)(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183053 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 44.91 KDa
Source organism: Janthinobacterium sp. J3
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84II6 (Residues: 1-384; Coverage: 100%)
Gene name: carAa
Sequence domains:
Structure domains:
Ferredoxin CarAc Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 115 amino acids
Theoretical weight: 12.45 KDa
Source organism: Pseudomonas resinovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GI16 (Residues: 1-107; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

2 bound ligands:
Ligand FE2 3 x FE2
Ligand FES 6 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 98.266Å b: 89.816Å c: 105.246Å
α: 90° β: 104.04° γ: 90°
R-values:
R R work R free
0.19 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Engineering Rieske oxygenase activity one piece at a time.
Brimberry et al. (2023)
6 other articles

1 mention without citation

Structural basis of the divergent oxygenation reactions catalyzed by the rieske nonheme iron oxygenase carbazole 1,9a-dioxygenase.
Inoue et al. (2014)