› 4n90

X-ray diffraction
3.3Å resolution

Crystal structure of ternary complex of TRAIL, DR5, and Fab fragment from a DR5 agonist antibody

Released:
DOI: 10.2210/pdb4n90/pdb
PDB entry 4n90 coloured by chain, front view.
PDB entry 4n90 coloured by chain, side view.
PDB entry 4n90 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity.
Graves JD, Kordich JJ, Huang TH, Piasecki J, Bush TL, Sullivan T, Foltz IN, Chang W, Douangpanya H, Dang T, O'Neill JW, Mallari R, Zhao X, Branstetter DG, Rossi JM, Long AM, Huang X, Holland PM
Cancer Cell 26 177-89 (2014)
PMID: 25043603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208024 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tumor necrosis factor receptor superfamily member 10B Chains: R, S, T
Molecule details ›
Chains: R, S, T
Length: 126 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14763 (Residues: 57-182; Coverage: 33%)
Gene names: DR5, KILLER, TNFRSF10B, TRAILR2, TRICK2, UNQ160/PRO186, ZTNFR9
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2
Tumor necrosis factor ligand superfamily member 10 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 168 amino acids
Theoretical weight: 19.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50591 (Residues: 114-281; Coverage: 60%)
Gene names: APO2L, TNFSF10, TRAIL
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Fab light chain Chains: E, G, I
Molecule details ›
Chains: E, G, I
Length: 215 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Fab heavy chain Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 224 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6122
Unit cell:
a: 152.006Å b: 152.006Å c: 613.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

34 review citations

Targeting apoptosis in cancer therapy.
Carneiro et al. (2020)
33 more

5 mentions without citation

Current Challenges and Opportunities in Designing Protein-Protein Interaction Targeted Drugs.
Shin et al. (2020)
4 more