› 4n7k

X-ray diffraction
2.85Å resolution

Zinc Substituted Reaction Center of the Rhodobacter sphaeroides

Released:
DOI: 10.2210/pdb4n7k/pdb
PDB entry 4n7k coloured by chain, front view.
PDB entry 4n7k coloured by chain, side view.
PDB entry 4n7k coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Structural and kinetic properties of Rhodobacter sphaeroides photosynthetic reaction centers containing exclusively Zn-coordinated bacteriochlorophyll as bacteriochlorin cofactors.
Saer RG, Pan J, Hardjasa A, Lin S, Rosell F, Mauk AG, Woodbury NW, Murphy ME, Beatty JT
Biochim Biophys Acta 1837 366-74 (2014)
PMID: 24316146

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 241 amino acids
Theoretical weight: 26.12 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 11-251; Coverage: 93%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 303 amino acids
Theoretical weight: 34 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-304; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

11 bound ligands:
Ligand GOL 7 x GOL
Ligand GGD 1 x GGD
Ligand 2GO 6 x 2GO
Ligand LDA 5 x LDA
Ligand U10 2 x U10
Ligand PO4 1 x PO4
Ligand HTO 2 x HTO
Ligand FE 1 x FE
Ligand SPO 1 x SPO
Ligand CDL 1 x CDL
Ligand PC1 1 x PC1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3121
Unit cell:
a: 139.45Å b: 139.45Å c: 184.181Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.243
Expression system: Cereibacter sphaeroides

Quick links

Citations

3 citation in other articles

Cardiolipin Interactions with Proteins.
Planas-Iglesias et al. (2015)
2 more

1 mention without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)