› 4n0u

X-ray diffraction
3.8Å resolution

Ternary complex between Neonatal Fc receptor, serum albumin and Fc

Released:
DOI: 10.2210/pdb4n0u/pdb
PDB entry 4n0u coloured by chain, front view.
PDB entry 4n0u coloured by chain, side view.
PDB entry 4n0u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into neonatal Fc receptor-based recycling mechanisms.
Oganesyan V, Damschroder MM, Cook KE, Li Q, Gao C, Wu H, Dall'Acqua WF
J Biol Chem 289 7812-24 (2014)
PMID: 24469444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-134850 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
IgG receptor FcRn large subunit p51 Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55899 (Residues: 27-290; Coverage: 77%)
Gene names: FCGRT, FCRN
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Albumin Chain: D
Molecule details ›
Chain: D
Length: 583 amino acids
Theoretical weight: 66.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02768 (Residues: 27-609; Coverage: 99%)
Gene names: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341
Sequence domains: Serum albumin family
Structure domains: Serum Albumin; Chain A, Domain 1
Immunoglobulin heavy constant gamma 1 Chain: E
Molecule details ›
Chain: E
Length: 209 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01857 (Residues: 119-327; Coverage: 52%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 153.19Å b: 153.19Å c: 145.998Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.282 0.303
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Molecular properties of human IgG subclasses and their implications for designing therapeutic monoclonal antibodies against infectious diseases.
Irani et al. (2015)
33 more

32 mentions without citation

Unraveling the Interaction between FcRn and Albumin: Opportunities for Design of Albumin-Based Therapeutics.
Sand et al. (2014)
31 more