› 4myf

X-ray diffraction
1.8Å resolution

Crystal structure of Trypanosoma cruzi formiminoglutamase(oxidized) with Mn2+2 at pH 6.0

Released:
DOI: 10.2210/pdb4myf/pdb
PDB entry 4myf coloured by chain, front view.
PDB entry 4myf coloured by chain, side view.
PDB entry 4myf coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme.
Hai Y, Dugery RJ, Healy D, Christianson DW
Biochemistry 52 9294-309 (2013)
PMID: 24261485

Function and Biology Details

Reaction catalysed: 
N-formimidoyl-L-glutamate + H(2)O = L-glutamate + formamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase, putative Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.64 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4DSA0 (Residues: 1-308; Coverage: 100%)
Gene name: Tc00.1047053507031.90
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 129.548Å b: 129.548Å c: 42.665Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.21
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Trypanosomatid Infections: How Do Parasites and Their Excreted-Secreted Factors Modulate the Inducible Metabolism of l-Arginine in Macrophages?
Holzmuller et al. (2018)
3 other articles

1 mention without citation

Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme.
Hai et al. (2013)