› 4mu4

X-ray diffraction
1.41Å resolution

The form B structure of an E21Q catalytic mutant of A. thaliana IGPD2 in complex with Mn2+ and its substrate, 2R3S-IGP, to 1.41 A resolution

Released:
DOI: 10.2210/pdb4mu4/pdb
PDB entry 4mu4 coloured by chain, front view.
PDB entry 4mu4 coloured by chain, side view.
PDB entry 4mu4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal Structures Reveal that the Reaction Mechanism of Imidazoleglycerol-Phosphate Dehydratase Is Controlled by Switching Mn(II) Coordination.
Bisson C, Britton KL, Sedelnikova SE, Rodgers HF, Eadsforth TC, Viner RC, Hawkes TR, Baker PJ, Rice DW
Structure 23 1236-45 (2015)
PMID: 26095028

Function and Biology Details

Reaction catalysed: 
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazoleglycerol-phosphate dehydratase 2, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.39 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O23346 (Residues: 69-272; Coverage: 75%)
Gene names: At4g14910, HISN5B, dl3495c
Sequence domains: Imidazoleglycerol-phosphate dehydratase
Structure domains: Imidazole glycerol phosphate dehydratase; domain 1

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand IYP 1 x IYP
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P432
Unit cell:
a: 112.9Å b: 112.9Å c: 112.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.123 0.154
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
2 more

4 mentions without citation

Crystal Structures Reveal that the Reaction Mechanism of Imidazoleglycerol-Phosphate Dehydratase Is Controlled by Switching Mn(II) Coordination.
Bisson et al. (2015)
3 more