› 4mqw

X-ray diffraction
2.9Å resolution

Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor (P31)

Released:
DOI: 10.2210/pdb4mqw/pdb
PDB entry 4mqw coloured by chain, front view.
PDB entry 4mqw coloured by chain, side view.
PDB entry 4mqw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evidence for Follicle-stimulating Hormone Receptor as a Functional Trimer.
Jiang X, Fischer D, Chen X, McKenna SD, Liu H, Sriraman V, Yu HN, Goutopoulos A, Arkinstall S, He X
J Biol Chem 289 14273-82 (2014)
PMID: 24692546

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134113 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycoprotein hormones alpha chain Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 102 amino acids
Theoretical weight: 11.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 25-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Structure domains: Cystine-knot cytokines
Follitropin subunit beta Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 111 amino acids
Theoretical weight: 12.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01225 (Residues: 19-129; Coverage: 100%)
Gene name: FSHB
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
Follicle-stimulating hormone receptor Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 361 amino acids
Theoretical weight: 41.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P23945 (Residues: 16-366; Coverage: 52%)
Gene names: FSHR, LGR1
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

3 bound ligands:
Ligand NAG 15 x NAG
Ligand EDO 5 x EDO
Ligand JEF 1 x JEF
1 modified residue:
Ligand TYS 3 x TYS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P31
Unit cell:
a: 95.9Å b: 95.9Å c: 204.281Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.237
Expression system: Homo sapiens

Quick links

Citations

26 review citations

Graves' disease.
Davies et al. (2020)
25 more

2 mentions without citation

In a Class of Their Own - RXFP1 and RXFP2 are Unique Members of the LGR Family.
Petrie et al. (2015)
1 more