› 4mnf

X-ray diffraction
2.8Å resolution

Crystal structure of BRAF-V600E bound to GDC0879

Released:
DOI: 10.2210/pdb4mnf/pdb
PDB entry 4mnf coloured by chain, front view.
PDB entry 4mnf coloured by chain, side view.
PDB entry 4mnf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the BRAF-MEK complex reveals a kinase activity independent role for BRAF in MAPK signaling.
Haling JR, Sudhamsu J, Yen I, Sideris S, Sandoval W, Phung W, Bravo BJ, Giannetti AM, Peck A, Masselot A, Morales T, Smith D, Brandhuber BJ, Hymowitz SG, Malek S
Cancer Cell 26 402-413 (2014)
PMID: 25155755

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P15056 (Residues: 432-736; Coverage: 40%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 29L 2 x 29L
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 99.74Å b: 99.74Å c: 159.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.211 0.274
Expression system: Trichoplusia ni

Quick links

Citations

23 review citations

Regulation of RAF protein kinases in ERK signalling.
Lavoie et al. (2015)
22 more

22 mentions without citation

Emerging BRAF Mutations in Cancer Progression and Their Possible Effects on Transcriptional Networks.
Śmiech et al. (2020)
21 more