› 4mfp

X-ray diffraction
2.15Å resolution

The crystal structure of acyltransferase in complex with decanoyl-CoA and Tei pseudoaglycone

Released:
DOI: 10.2210/pdb4mfp/pdb
PDB entry 4mfp coloured by chain, front view.
PDB entry 4mfp coloured by chain, side view.
PDB entry 4mfp coloured by chain, top view.
Source organism: Actinoplanes teichomyceticus
Primary publication:
Multiple complexes of long aliphatic N-acyltransferases lead to synthesis of 2,6-diacylated/2-acyl-substituted glycopeptide antibiotics, effectively killing vancomycin-resistant enterococcus.
Lyu SY, Liu YC, Chang CY, Huang CJ, Chiu YH, Huang CM, Hsu NS, Lin KH, Wu CJ, Tsai MD, Li TL
J Am Chem Soc 136 10989-95 (2014)
PMID: 25095906

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164817 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 39.2 KDa
Source organism: Actinoplanes teichomyceticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70AY4 (Residues: 1-323; Coverage: 100%)
Gene names: FHX34_108182, tcp24
Sequence domains:
Structure domains: Aminopeptidase
Teicoplanin pseudoaglycone Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 1.21 KDa
Source organism: Actinoplanes teichomyceticus

Ligands and Environments

6 bound ligands:
Ligand COA 1 x COA
Ligand SO4 2 x SO4
Ligand NAG 1 x NAG
Ligand MAN 1 x MAN
Ligand GCS 1 x GCS
Ligand DKA 1 x DKA
3 modified residues:
Ligand GHP 3 x GHP
Ligand 3MY 1 x 3MY
Ligand OMY 1 x OMY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A
Spacegroup: P65
Unit cell:
a: 134.107Å b: 134.107Å c: 49.445Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent advances in engineering nonribosomal peptide assembly lines.
Winn et al. (2016)
2 more