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REFINED CRYSTAL STRUCTURE OF CYTOPLASMIC MALATE DEHYDROGENASE AT 2.5-ANGSTROMS RESOLUTION

Source organism: Sus scrofa
Biochemical function: NAD binding
Biological process: carboxylic acid metabolic process
Cellular component: cytosol

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EC 1.1.1.96: Diiodophenylpyruvate reductase

Reaction catalysed:
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD(+) = 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
Systematic name:
3-(3,5-diiodo-4-hydroxyphenyl)lactate:NAD(+) oxidoreductase
Alternative Name(s):
  • 2-oxo acid reductase
  • Aromatic alpha-keto acid
  • KAR

EC 1.1.1.37: Malate dehydrogenase

Reaction catalysed:
(S)-malate + NAD(+) = oxaloacetate + NADH
Systematic name:
(S)-malate:NAD(+) oxidoreductase
Alternative Name(s):
  • L-malate dehydrogenase
  • L-malate-NAD(+) oxidoreductase
  • MDH
  • Malate (NAD) dehydrogenase
  • Malic acid dehydrogenase
  • Malic dehydrogenase
  • NAD-L-malate dehydrogenase
  • NAD-dependent malate dehydrogenase
  • NAD-dependent malic dehydrogenase
  • NAD-linked malate dehydrogenase
  • NAD-malate dehydrogenase
  • NAD-malic dehydrogenase
  • NAD-specific malate dehydrogenase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF02866
Domain description: lactate/malate dehydrogenase, alpha/beta C-terminal domain
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of Pfam domain PF02866 (lactate/malate dehydrogenase, alpha/beta C-terminal domain) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.

PF00056
Domain description: lactate/malate dehydrogenase, NAD binding domain
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of Pfam domain PF00056 (lactate/malate dehydrogenase, NAD binding domain) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR010945
Domain description: Malate dehydrogenase, type 2
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR015955
Domain description: Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR011274
Domain description: Malate dehydrogenase, NAD-dependent, cytosolic
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR022383
Domain description: Lactate/malate dehydrogenase, C-terminal
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR001252
Domain description: Malate dehydrogenase, active site
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR001236
Domain description: Lactate/malate dehydrogenase, N-terminal
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR036291
Domain description: NAD(P)-binding domain superfamily
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
IPR001557
Domain description: L-lactate/malate dehydrogenase
Occurring in:
  1. Malate dehydrogenase, cytoplasmic

Structure domains

CATH CATH domains
3.90.110.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: L-2-Hydroxyisocaproate Dehydrogenase; Chain A, domain 2
Homology: Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of CATH domain 3.90.110.10 (L-2-Hydroxyisocaproate Dehydrogenase; Chain A, domain 2) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.
3.40.50.720
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: NAD(P)-binding Rossmann-like Domain
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of CATH domain 3.40.50.720 (Rossmann fold) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.
SCOP SCOP annotations
56328
Class: Alpha and beta proteins (a+b)
Fold: LDH C-terminal domain-like
Superfamily: LDH C-terminal domain-like
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of SCOP domain 56328 (Lactate & malate dehydrogenases, C-terminal domain) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.
51848
Class: Alpha and beta proteins (a/b)
Fold: NAD(P)-binding Rossmann-fold domains
Superfamily: NAD(P)-binding Rossmann-fold domains
Occurring in:
  1. Malate dehydrogenase, cytoplasmic
The deposited structure of PDB entry 4mdh contains 2 copies of SCOP domain 51848 (LDH N-terminal domain-like) in Malate dehydrogenase, cytoplasmic. Showing 1 copy in chain A.

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