4m95

X-ray diffraction
1.72Å resolution

d(ATCCGTTATAACGGAT)complexed with Moloney Murine Leukemia virus reverse transcriptase catalytic fragment

Released:

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117834 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Reverse transcriptase/ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.35 KDa
Source organism: Moloney murine leukemia virus isolate Shinnick
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 683-937; Coverage: 15%)
Gene name: gag-pol
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
5' d(ATCCGTTA) 3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Moloney murine leukemia virus isolate Shinnick
Expression system: Not provided
5' d(TAACGGAT) 3' Chain: G
Molecule details ›
Chain: G
Length: 8 nucleotides
Theoretical weight: 2.45 KDa
Source organism: Moloney murine leukemia virus isolate Shinnick
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 54.329Å b: 146.107Å c: 46.972Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.234
Expression systems:
  • Escherichia coli
  • Not provided