› 4m71

X-ray diffraction
2.21Å resolution

Mutant structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-homocysteine and methylphenylpyruvic acid

Released:
DOI: 10.2210/pdb4m71/pdb
PDB entry 4m71 coloured by chain, front view.
PDB entry 4m71 coloured by chain, side view.
PDB entry 4m71 coloured by chain, top view.
Source organism: Streptomyces hygroscopicus
Primary publication:
Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase.
Zou XW, Liu YC, Hsu NS, Huang CJ, Lyu SY, Chan HC, Chang CY, Yeh HW, Lin KH, Wu CJ, Tsai MD, Li TL
Acta Crystallogr D Biol Crystallogr 70 1549-60 (2014)
PMID: 24914966

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 3-phenylpyruvate = S-adenosyl-L-homocysteine + (3S)-2-oxo-3-phenylbutanoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylpyruvate C(3)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.2 KDa
Source organism: Streptomyces hygroscopicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q643C8 (Residues: 1-337; Coverage: 100%)
Gene name: mppJ
Sequence domains: Methyltransferase domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
5 bound ligands:
Ligand 56D 2 x 56D
Ligand FE 2 x FE
Ligand HF2 2 x HF2
Ligand CA 14 x CA
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A
Spacegroup: P212121
Unit cell:
a: 60.833Å b: 93.693Å c: 137.375Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.158 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
4 more