› 4lyp

X-ray diffraction
1.28Å resolution

Crystal Structure of Glycoside Hydrolase Family 5 Mannosidase from Rhizomucor miehei

Released:
DOI: 10.2210/pdb4lyp/pdb
PDB entry 4lyp coloured by chain, front view.
PDB entry 4lyp coloured by chain, side view.
PDB entry 4lyp coloured by chain, top view.
Source organism: Rhizomucor miehei
Primary publication:
Structural insights into the substrate specificity and transglycosylation activity of a fungal glycoside hydrolase family 5 β-mannosidase.
Zhou P, Liu Y, Yan Q, Chen Z, Qin Z, Jiang Z
Acta Crystallogr D Biol Crystallogr 70 2970-82 (2014)
PMID: 25372687

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-beta-1,4-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 51.3 KDa
Source organism: Rhizomucor miehei
Expression system: Escherichia coli
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand GAI 4 x GAI
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 50.646Å b: 171.991Å c: 55.137Å
α: 90° β: 104.6° γ: 90°
R-values:
R R work R free
0.121 0.119 0.153
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mannoside recognition and degradation by bacteria.
Ladevèze et al. (2017)
15 other articles

2 mentions without citation

Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes.
Gómez-Silva et al. (2019)
1 more