› 4lkb

X-ray diffraction
2.16Å resolution

Crystal structure of a putative 4-Oxalocrotonate Tautomerase from Nostoc sp. PCC 7120

Released:
DOI: 10.2210/pdb4lkb/pdb
PDB entry 4lkb coloured by chain, front view.
PDB entry 4lkb coloured by chain, side view.
PDB entry 4lkb coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Al Obaidi N, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tautomerase enzyme Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 151 amino acids
Theoretical weight: 17.91 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YNJ7 (Residues: 1-129; Coverage: 100%)
Gene name: alr4568
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 111.022Å b: 132.926Å c: 133.121Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.176 0.221
Expression system: Escherichia coli BL21(DE3)

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