› 4lc1

X-ray diffraction
1.8Å resolution

MeaB, A Bacterial Homolog of MMAA, Bound to GDP and crystallized in the presence of GDP and [AlF4]-

Released:
DOI: 10.2210/pdb4lc1/pdb
PDB entry 4lc1 coloured by chain, front view.
PDB entry 4lc1 coloured by chain, side view.
PDB entry 4lc1 coloured by chain, top view.
Source organism: Methylorubrum extorquens AM1
Primary publication:
Autoinhibition and signaling by the switch II motif in the G-protein chaperone of a radical B12 enzyme.
Lofgren M, Koutmos M, Banerjee R
J Biol Chem 288 30980-9 (2013)
PMID: 23996001

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylmalonyl-CoA mutase accessory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 35.6 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5AP93 (Residues: 1-329; Coverage: 100%)
Gene names: MexAM1_META1p0188, meaB
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 63.667Å b: 78.623Å c: 69.622Å
α: 90° β: 108.61° γ: 90°
R-values:
R R work R free
0.196 0.179 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
13 other articles

2 mentions without citation

The requirement for cobalt in vitamin B12: A paradigm for protein metalation.
Osman et al. (2021)
1 more