› 4kw7

X-ray diffraction
1.8Å resolution

The structure of an As(III) S-adenosylmethionine methyltransferase with Phenylarsine oxide(PAO)

Released:
DOI: 10.2210/pdb4kw7/pdb
PDB entry 4kw7 coloured by chain, front view.
PDB entry 4kw7 coloured by chain, side view.
PDB entry 4kw7 coloured by chain, top view.
Source organism: Cyanidioschyzon sp. 5508
Primary publication:
A disulfide-bond cascade mechanism for arsenic(III) S-adenosylmethionine methyltransferase.
Marapakala K, Packianathan C, Ajees AA, Dheeman DS, Sankaran B, Kandavelu P, Rosen BP
Acta Crystallogr D Biol Crystallogr 71 505-15 (2015)
PMID: 25760600

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 41.8 KDa
Source organism: Cyanidioschyzon sp. 5508
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0JV69 (Residues: 1-370; Coverage: 93%)
Gene name: arsM
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand PA0 1 x PA0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 85.095Å b: 47.41Å c: 100.375Å
α: 90° β: 113.48° γ: 90°
R-values:
R R work R free
0.205 0.203 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

New mechanisms of bacterial arsenic resistance.
Yang et al. (2016)
5 more

8 mentions without citation

The organoarsenical biocycle and the primordial antibiotic methylarsenite.
Li et al. (2016)
7 more