› 4kto

X-ray diffraction
2.14Å resolution

Crystal Structure Of a Putative Isovaleryl-CoA dehydrogenase (PSI-NYSGRC-012251) from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb4kto/pdb
PDB entry 4kto coloured by chain, front view.
PDB entry 4kto coloured by chain, side view.
PDB entry 4kto coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Kumar PR, Ahmed M, Attonito J, Bhosle R, Bonanno J, Chamala S, Chowdhury S, Glenn AS, Hammonds J, Hillerich B, Himmel D, Love JD, Seidel R, Stead M, Toro R, Wasserman SR, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
Isovaleryl-CoA + electron-transfer flavoprotein = 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isovaleryl-CoA dehydrogenase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 410 amino acids
Theoretical weight: 44.75 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92VK1 (Residues: 2-387; Coverage: 100%)
Gene names: SM_b21121, ivdH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
Ligand TRS 3 x TRS
1 modified residue:
Ligand MSE 68 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 89.912Å b: 115.158Å c: 143.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.226
Expression system: Escherichia coli BL21(DE3)

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