Function and Biology Details
Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PCL10-PHO85 kinase complex (preferred)
PDBe Complex ID:
PDB-CPX-147880 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent protein kinase PHO85
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Protein kinase domain
Structure domains:
Length: 317 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P17157 (Residues: 1-305; Coverage: 100%)
Sequence domains: Protein kinase domain
Structure domains:
PHO85 cyclin-10
Molecule details ›
Chain: B
Length: 207 amino acids
Theoretical weight: 23.75 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Cyclin
Structure domains: Cyclin-like
Length: 207 amino acids
Theoretical weight: 23.75 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P53124 (Residues: 227-433; Coverage: 48%)
Sequence domains: Cyclin
Structure domains: Cyclin-like
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 22-ID
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
