› 4klz

X-ray diffraction
2.3Å resolution

Inhibition of Small GTPases by Stabilization of the GDP Complex, a Novel Approach applied to Rit1, a Target for Rheumatoid Arthritis

Released:
DOI: 10.2210/pdb4klz/pdb
PDB entry 4klz coloured by chain, front view.
PDB entry 4klz coloured by chain, side view.
PDB entry 4klz coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Shah DM, Kobayashi M, Keizers PH, Tuin AW, Ab E, Manning L, Rzepiela AA, Andrews M, Hoedemaeker FJ, Siegal G

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rit1 Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 20.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92963 (Residues: 19-189; Coverage: 78%)
Gene names: RIBB, RIT, RIT1, ROC1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 40.278Å b: 37.048Å c: 40.736Å
α: 90° β: 95.9° γ: 90°
R-values:
R R work R free
0.198 0.185 0.311
Expression system: Escherichia coli BL21(DE3)

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