4kah

X-ray diffraction
1.8Å resolution

Crystal structure of probable sugar kinase protein from Rhizobium etli CFN 42 complexed with 4-bromo-1H-pyrazole

Released:
Source organism: Rhizobium etli CFN 42
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-173687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 38.85 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KDX6 (Residues: 1-330; Coverage: 100%)
Gene name: RHE_CH00135
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 81.006Å b: 90.964Å c: 92.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.152 0.201
Expression system: Escherichia coli BL21(DE3)