› 4k9l

X-ray diffraction
1.65Å resolution

Crystal Structure of the His281Thr mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Released:
DOI: 10.2210/pdb4k9l/pdb
PDB entry 4k9l coloured by chain, front view.
PDB entry 4k9l coloured by chain, side view.
PDB entry 4k9l coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Brodkin HR, McLeish MJ

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 531 amino acids
Theoretical weight: 56.84 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20906 (Residues: 2-526; Coverage: 99%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TZD 1 x TZD
2 bound ligands:
Ligand CA 1 x CA
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 80.721Å b: 95.486Å c: 137.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.187
Expression system: Escherichia coli BL21(DE3)

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