› 4k7w

X-ray diffraction
1.76Å resolution

Crystal structure of Zn3-hUb(human ubiquitin) adduct from a solution 100 mM zinc acetate/1.3 mM hUb

Released:
DOI: 10.2210/pdb4k7w/pdb
PDB entry 4k7w coloured by chain, front view.
PDB entry 4k7w coloured by chain, side view.
PDB entry 4k7w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational selection of ubiquitin quaternary structures driven by zinc ions.
Fermani S, Falini G, Calvaresi M, Bottoni A, Calò V, Mangini V, Arnesano F, Natile G
Chemistry 19 15480-4 (2013)
PMID: 24123543

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand ZN 9 x ZN
Ligand ACT 3 x ACT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 43.88Å b: 50.51Å c: 93.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.267
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Exploring CRD mobility during RAS/RAF engagement at the membrane.
Nguyen et al. (2022)
2 more

1 mention without citation

New crystal form of human ubiquitin in the presence of magnesium.
Camara-Artigas et al. (2016)