› 4k3c

X-ray diffraction
2.91Å resolution

The crystal structure of BamA from Haemophilus ducreyi lacking POTRA domains 1-3

Released:
DOI: 10.2210/pdb4k3c/pdb
PDB entry 4k3c coloured by chain, front view.
PDB entry 4k3c coloured by chain, side view.
PDB entry 4k3c coloured by chain, top view.
Source organism: [Haemophilus] ducreyi
Primary publication:
Structural insight into the biogenesis of β-barrel membrane proteins.
Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK
Nature 501 385-90 (2013)
PMID: 23995689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 532 amino acids
Theoretical weight: 59.43 KDa
Source organism: [Haemophilus] ducreyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93PM2 (Residues: 262-793; Coverage: 69%)
Gene names: D15, RZ57_04770, bamA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 47.779Å b: 88.162Å c: 244.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.27
Expression system: Escherichia coli

Quick links

Citations

60 review citations

The antimicrobial activity of nanoparticles: present situation and prospects for the future.
Wang et al. (2017)
59 more

23 mentions without citation

The β-barrel membrane protein insertase machinery from Gram-negative bacteria.
Noinaj et al. (2015)
22 more