› 4jzz

X-ray diffraction
1.49Å resolution

Crystal structure of CD4-mimetic miniprotein M48U1 in complex with HIV-1 YU2 gp120 in C2221 space group

Released:
DOI: 10.2210/pdb4jzz/pdb
PDB entry 4jzz coloured by chain, front view.
PDB entry 4jzz coloured by chain, side view.
PDB entry 4jzz coloured by chain, top view.
Source organism: Human immunodeficiency virus 1
Primary publication:
Structural basis for highly effective HIV-1 neutralization by CD4-mimetic miniproteins revealed by 1.5 Å cocrystal structure of gp120 and M48U1.
Acharya P, Luongo TS, Louder MK, McKee K, Yang Y, Kwon YD, Mascola JR, Kessler P, Martin L, Kwong PD
Structure 21 1018-29 (2013)
PMID: 23707685

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153067 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Surface protein gp120 Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 41.7 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: P35961 (Residues: 21-479; Coverage: 45%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
CD4-MIMETIC MINIPROTEIN M48U1 Chain: R
Molecule details ›
Chain: R
Length: 28 amino acids
Theoretical weight: 3.06 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand NAG 8 x NAG
Ligand NA 6 x NA
Ligand IPA 5 x IPA
Ligand FLC 1 x FLC
2 modified residues:
Ligand DPR 1 x DPR
Ligand U2X 1 x U2X

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 65.087Å b: 164.719Å c: 78.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.189
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

5 review citations

Conformational Masking and Receptor-Dependent Unmasking of Highly Conserved Env Epitopes Recognized by Non-Neutralizing Antibodies That Mediate Potent ADCC against HIV-1.
Lewis et al. (2015)
4 more

7 mentions without citation

HIV-1 gp120 as a therapeutic target: navigating a moving labyrinth.
Acharya et al. (2015)
6 more