› 4jxu

X-ray diffraction
2.4Å resolution

Structure of aminotransferase ilvE2 from Sinorhizobium meliloti complexed with PLP

Released:
DOI: 10.2210/pdb4jxu/pdb
PDB entry 4jxu coloured by chain, front view.
PDB entry 4jxu coloured by chain, side view.
PDB entry 4jxu coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Cooper DR, Cymborowski MT, Majorek KA, Niedzialkowska E, Porebski PJ, Stead M, Hammonds J, Seidel R, Ahmed M, Bonanno JB, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable branched-chain-amino-acid aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 33.03 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92RC2 (Residues: 2-293; Coverage: 100%)
Gene names: SMc00042, ilvE2
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 22 x MSE
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 98.799Å b: 76.006Å c: 93.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.212
Expression system: Escherichia coli BL21(DE3)

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