4jv4 Summary


Crystal Structure of RIalpha(91-379) bound to HE33, a N6 di-propyl substituted cAMP analog

The structure was published by Brown, S.H., Cheng, C.Y., Saldanha, S.A., et al., Cottam, H.B., Sankaran, B., and Taylor, S.S., in 2013 in a paper entitled "Implementing Fluorescence Anisotropy Screening and Crystallographic Analysis to Define PKA Isoform-Selective Activation by cAMP Analogs." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.952 Å and deposited in 2013.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of cAMP-dependent protein kinase type I-alpha regulatory subunit. This molecule has the UniProt identifier P00514 (KAP0_BOVIN)search. The sample contained 288 residues which is < 90% of the natural sequence. Out of 288 residues 250 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A cAMP-dependent protein kinase type I-alpha regulatory subunit P00514 (93-377) (KAP0_BOVIN)search Bos taurussearch 100% 288 90%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00514 (93 - 377) cAMP-dependent protein kinase type I-alpha regulatory subunit Bos taurus

Chain Sequence family (Pfam)
A (P00514) PF00027: Cyclic nucleotide-binding domainsearch

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
A (P00514) cAMP-dependent protein kinase complexsearch regulation of protein phosphorylationsearch cAMP-dependent protein kinase regulator activitysearch

Chain InterPro annotation
A Cyclic nucleotide-binding domainsearch cAMP/cGMP-dependent protein kinasesearch cAMP-dependent protein kinase regulatory subunitsearch RmlC-like jelly roll foldsearch Cyclic nucleotide-binding, conserved sitesearch Cyclic nucleotide-binding-likesearch