› 4jri

X-ray diffraction
1.83Å resolution

Crystal Structure of Escherichia coli Hfq Proximal Edge Mutant

Released:
DOI: 10.2210/pdb4jri/pdb
PDB entry 4jri coloured by chain, front view.
PDB entry 4jri coloured by chain, side view.
PDB entry 4jri coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Mapping Hfq-RNA interaction surfaces using tryptophan fluorescence quenching.
Robinson KE, Orans J, Kovach AR, Link TM, Brennan RG
Nucleic Acids Res 42 2736-49 (2014)
PMID: 24288369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 68 amino acids
Theoretical weight: 7.67 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0A6X3 (Residues: 2-69; Coverage: 67%)
Gene names: JW4130, b4172, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P213
Unit cell:
a: 90.087Å b: 90.087Å c: 90.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.257
Expression system: Not provided

Quick links

Citations

10 review citations

Hfq: the flexible RNA matchmaker.
Updegrove et al. (2016)
9 more

1 mention without citation

An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification.
Zhou et al. (2021)