› 4jqo

X-ray diffraction
2.08Å resolution

Crystal structure of anabolic ornithine carbamoyltransferase from Vibrio vulnificus in complex with citrulline and inorganic phosphate

Released:
DOI: 10.2210/pdb4jqo/pdb
PDB entry 4jqo coloured by chain, front view.
PDB entry 4jqo coloured by chain, side view.
PDB entry 4jqo coloured by chain, top view.
Source organism: Vibrio vulnificus CMCP6
Entry authors: Shabalin IG, Winsor J, Grimshaw S, Domagalski MJ, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine carbamoyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 358 amino acids
Theoretical weight: 39.64 KDa
Source organism: Vibrio vulnificus CMCP6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8DCF5 (Residues: 1-334; Coverage: 100%)
Gene names: VV1_1466, argF
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

4 bound ligands:
Ligand PO4 3 x PO4
Ligand CIR 3 x CIR
Ligand CL 3 x CL
Ligand PEG 7 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 81.418Å b: 82.597Å c: 171.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.189
Expression system: Escherichia coli BL21

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